Nigerian Journal of Microbiology,2009, Vol., 23(1);1817 - 1822
The Microbial Associates Of Unexpired And Expired Paediatric Syrups.
1Ogbulie J.N.; 2*Ibe, I.J and 1Nguma, C. C.
1Department of Microbiology,Federal University of Technology,,Owerri, Nigeria. 2Department of Biology\Microbiology,Federal Polytechnic, Nekede, Owerri, ,Nigeria.
Abstract
The microbial isolates of unstable and stable vitamin B-complex, Ventoiln, Colipan,
Revitone, and KP-Multivitamin syrups sold in Orlu main market were carried out. The total heterotrophic bacterial count, were 2.0 x 102, 0.3 x 102,1.0 x 102, 2.1 x 102, 3.3 x 102, 0.8 x 102, 3.7x102 cfu|ml while fungi were 1.0 x 102, 0.6 x 102,0.2 x 102,1.3 x 102, 2.0 x 102, 0.4 x 102,0.2 x 102, 0.0 x 102 cfu|ml for vitamin B-complex,Ventolin, Coilpan, Revitone, and KP-Multivitamin respectively. The study revealed the isolation of Staphylococcus, Micrococcus,Escherichia coli, Bacillus, Aspergillus, Penicillum and Rhizopus. The presence of isolates are indicative possible problems that can be associated with the consumption of any of these expired products.
Key words: microbial associates, total heterotrophic bacteria, isolates Corresponding author ; Email: aijaybaby2002@yahoo.co.uk 08035990380.
Introduction
the treatment of diseases (Eka et al.,
sterile liquid products were examined for
syrups, solutions, off-tonics, elixirs and
isolated include E. coli, Pseudomonas
species and Klebsiella species; while S.
children and infants: groups of patients
products to these people pose real danger,
(Mendie et al., 1993; Betram, 2004; Itah
Taylor et al., (2001) also reported that
Ogbulie et al., 1998; Prescott et al.,Nig. J. Microbiol., 23(1);1817 -1822
often prepared for children. Dilution in
Isolation identification bacteria and fungi
diluents. After serial dilution, 0.1ml of
the dilution was plated out using spread
plated out in duplicates on nutrient agar
them inactive or toxic (Ghulan et al.,
Samonella and Shigella species, and
agents are introduced with the intention
biochemical analysis for characterization
heterotrophic count of bacteria and fungi.
2.1 x 102, 2.0 x 102 and 1.0 x102 cfu|ml
Mendie et al., 1993, Betram, 2004).
Materials and methods Nig. J. Microbiol., 23(1);1817 -1822
isolates. For bacterial, Staphylocoous, Bacillus and Micrococcus accounted for
0.6 x 102 cfu\ml and unexpired Ventolin,
Penicillium,Rhizopus and Aspergillus Table1: Total Heterotrophic Count of Bacteria\Fungi. Table 2: Percentage occurrence of different genera
Key: Staph = Staph. aureus, Bac = Bacillus species, Micro = Micrococcus species,
Rhi=Rhizopus species, Pen=Penicillium species, Asp= Aspergillus species
Nig. J. Microbiol., 23(1);1817 - 1822 Discussion
unexpired paediatric syrups sold in Orlu
(Mendie et al., 1993, Eka et al., 1987).
total heterotrophic bacterial and fungal
3.7 x 102 cfu|ml (for bacteria) and 0.2 x
102 to 2.0 x 102 cfu|ml (for fungi). These
findings are similar those of Hossain et al., (2004). Expired drug samples had
could be attributed to lack of appropriate
recommends Salmonella, Pseudomonas aeruginosa, E. coli and Staph.aureus as
indicators of microbial contamination of
Research, 2000). Staph. aureus was
isolated from syrup samples in this study.
It had the highest percentage occurrence
of all the genera (27.3%). Its isolation,
neonates, children and infant, groups of
along with Bacillus spp, has health
isolates in stable syrups collaborates the
report of Hossain et al., (2004). Nahata,
and use (Itah, et al., 2004). The
poor personal hygiene, low efficiency of
inspection of drugs should be carried out
standards (Panusa and Gargliardi, 2008).
Nig. J. Microbiol., 23(1);1817 - 1822References
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Holt, J.G ( 1984 ) Shorter Bergey’s
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