Welcome to the Spring 2004 Edition of InfoLink. The feature article in this edition has been
written by Dr Rodger Laurent, Head of Department, PaLMS Rheumatology Laboratory.
The article explores Anti-neutrophil cytoplasmic antibodies (ANCA) and the role ANCA
plays in the diagnosis and ongoing treatments of a small subgroup of vasculitides, which
have been designated the ANCA associated vasculitides.
It is with pride that we announce that PaLMS Director, Professor Leslie Burnett, has
been honoured by the American Association of Clinical Chemistry with the Management
Sciences Division Award. This award is in recognition of his “pioneering work to make
pathology and laboratory testing more accurate”. Prof Burnett is the ﬁrst non-American
to claim a top scientiﬁc award from this association.
For some years now PaLMS has used Telstra X400 for electronic delivery of reports.
PaLMS has commenced delivery of electronic pathology reports via HealthLink and are
phasing out the use of the Telstra X400. HealthLink is an established provider of delivery
services for health related data throughout New Zealand and East Coast Australia.
If there is one area that seems to exasperate requesting practitioners, it is the effort one
often has to go to meet the obligations under the Health Insurance Act 1973. This is
particularly true in relation to valid requests for pathology services. This issue contains a
ﬂyer regarding our reciprocal obligations under the act and the processes PaLMS has put
in place to assist us to meet our mutual obligations.
The Royal College of Pathologists of Australasia has launched a new magazine PathWay.
A copy of the new and informative publication accompanies this issue of InfoLink. If
you would like additional copies of PathWay for your patients, staff or colleagues please
contact PaLMS Administration on 9926 8086. PaLMS are pleased to be able to support
If there is a topic you wish covered in future issues or you have any feedback relating to
this issue, please contact a member of the editorial team whose details appear below. Editorial Team
Or send a fax c/- PaLMS Administration Fax: 9926-6395
Anti-Neutrophil Cytoplasmic Antibodies - ANCA Dr Rodger Laurent, Senior Staff Specialist Head - Department of Rheumatology Immuno-Rheumatology Laboratory, PaLMS What are anti-neutrophil cytoplasmic antibodies?
Anti-neutrophil cytoplasmic antibodies (ANCA) are antibodies directed against several cytoplasmic
antigens in neutrophils and monocytes. ANCA are associated only with a small subgroup of vasculitides,
which predominantly involve small sized vessels. This subgroup have been designated the ANCA
associated vasculitides. Most of the antigens are in the primary granules and the majority have antibacterial properties.
They can also be expressed on the surface of stimulated or apoptotic neutrophils but not on resting
neutrophils. There are several known target antigens of ANCA. These are proteinase 3, myeloperoxidase, elastase,
cathepsin G, azurocidin, lactoferrin, lysozyme and bacterial permeability-increasing protein. At present
only proteinaise-3 (PR3) and myeloperoxidase (MPO) are clinically relevant and routinely measured. ANCA probably have a pathogenetic role although its mechanism of action is unknown. What vasculitides are associated with ANCA?
Wegener’s granulomatosisMicroscopic polyangiitisPauci-immune crescentic glomerulonephritisChurg-Strauss syndrome
The common pathology in these diseases is predominant involvement of capillaries and venules as well
as arterioles. There is early invasion of the vessel wall with neutrophils which may be the cause of vessel
wall damage and necrosis. There is no association of ANCA with other vasculitides. These include polyarteritis, leukocytoclastic
vasculitis, giant cell arteritis, Kawasaki disease, Takayasu’s arteritis and Behcet’s syndrome. ANCA may
be present in a small percentage of these vasculitides but the association is too weak to be of any clinical
relevance. Wegener’s granulomatosis:
Wegener’s granulomatosis is characterised by inﬂammatory destructive lesions that predominantly aﬀect
the sinuses, ears, trachea, bronchi and lungs. Renal disease manifests as a glomerulonephritis. It may
also aﬀect the eyes and the joints. The strongest association between ANCA and a disease is between C-ANCA with Wegener’s
granulomatosis. ANCA is present in about 90% of those with active disease and the target antigen is
usually PR3. In 10% it can be a P-ANCA with the target antigen being MPO. Microscopic Polyangiitis
Microscopic polyangiitis involves predominantly the lungs and kidney. There may also be secondary
pulmonary haemorrhage. Renal involvement consists of a glomerulonephritis. Mononeuritis multiplex
can also occur but is less common than in other vasculitides.
ANCA is present in 40-80% of patients with microscopic polyangiitis. It is usually the P-ANCA
pattern and the target antigen is MPO. Pauci-Immune Crescentic Glomerulonephritis
This is probably part of the Wegener’s granulomatosis/microscopic polyangiitis spectrum. It is a
vasculitis in which there is minimal inﬂammatory cell inﬁltrate in the kidney. About 75-80% have a P-ANCA pattern with antibodies against MPO. Churg-Strauss Syndrome
Churg-Strauss vasculitis predominantly aﬀects the respiratory tract. It is associated with asthma and
allergic rhinitis. It produces fever, weight loss, lung involvement and mononeuritis multiplex. There is
often a peripheral blood eosinophilia and eosinophils deposited in the vasculitic lesions. ANCA is present in about 60% of patients with Churg-Strauss syndrome. The target antigen can be
either PR3 in 30% or MPO in 30% of cases. How is ANCA measured?
Measurement of ANCA is by indirect immunoﬂuorescence (IIF) and an enzyme linked immunoassay
(ELISA). Indirect immunoﬂuorescence:
• Indirect immunoﬂuorescence on neutrophils is used as the initial screen for ANCA. • There are two main staining patterns C-ANCA and P-ANCA. The C-ANCA pattern is where
the cytoplasm is diﬀusely stained and the P-ANCA pattern is where the staining is predominantly
• The C-ANCA pattern is usually associated with antibodies to the serine protease, proteinase 3
(PR3). This pattern can occasionally be associated with antibodies to MPO.
• The P-ANCA pattern is usually associated with antibodies to myeloperoxidase (MPO). • The P-ANCA pattern can occur in the absence of antibodies to MPO. This is because the P-ANCA
pattern can be associated with other antigens, for example, elastase and lactoferrin.
• A pattern which is diﬀerent to the C-ANCA or P-ANCA patterns is called atypical ANCA. These
sera have antibodies to other neutrophil antigens which include nuclear, cytoplasmic and granular
• Antinuclear antibodies (ANA) can react with neutrophils in the indirect immunoﬂuorescence assay
making it diﬃcult to interpret. In this situation it is essential to measure antibodies to PR3 and
Enzyme linked immunoassay:
• The ELISA assay is used to determine the speciﬁcity of the target antigen. The speciﬁc antigens
• If there is a positive ANCA with a particular staining pattern then it is important to test for the
• A recently described capture enzyme immunoassay may be more sensitive and have a better
When should I request an ANCA
• There is no value in using ANCA as a general screening test for vasculitis in patients with a vague
• It is only going to be of diagnostic value in patients with any of the following clinical abnormalities:
Proteinuria and haematuriaAcute or chronic renal failureHaemoptysisAbnormal chest x rayCutaneous vasculitis, particularly if there are associated systemic featuresMononeuritis multiplex or peripheral neuropathyChronic upper airways disease, particularly chronic sinusitisChronic iritis or retro-orbital swelling
• An ANCA should not be regarded as a diagnostic test. It is a useful aid to diagnosis and monitoring
• Vasculitis should always be diagnosed on the histopathological ﬁndings in a biopsy of the involved
• A biopsy is essential so that non-vasculitic disorders in which ANCA may occur are not
inappropriately treated. These include connective tissue disorders, inﬂammatory bowel disease,
infections, tuberculosis and bacterial endocarditis, malignancy and medications. Why should I only order an ANCA if there are speciﬁc clinical features?
• The ANCA associated vasculitides are rare so the pretest probability of disease is very low. This
results in a large number of false positives with a poor diagnostic yield for ANCA testing.
• The performance of the test can be improved by increasing the pretest probability. This is done
by only ordering ANCA in patients with a high likelihood of the disease. They have the clinical
features previously listed in the preceding section.
• Using the test in the appropriate clinical context reduces the number of false positives without
missing any cases of ANCA associated vasculitis. How does ANCA help with the diagnosis of vasculitis?
• Both the IIF and corresponding ELISA must be positive to support the diagnosis of an ANCA
• The clinical value of either test alone being positive is limited, providing little help with the
• Therefore, a positive result from the IIF method should always be conﬁrmed with an antigen
• The combination of IIF and ELISA assays for PR3 and MPO improve the speciﬁcity and sensitivity
• If these criteria are met there is a speciﬁcity of 99% for an ANCA associated vasculitis, with a
sensitivity of 73% for Wegener’s granulomatosis and 67% for microscopic polyangiitis.
• It is important to remember that a negative ANCA does not rule out a diagnosis of vasculitis. What does the ANCA report tell me?
The following is an example of the format of a laboratory report for ANCA:
Anti-neutrophil cyto. Ab POSITIVE. C-ANCA
This report indicates that there are antibodies against neutrophil cytoplasmic components. They
produced the C-ANCA pattern on immunoﬂuorescence with a titre of 1:160. This is due to antibodies
against PR3, which are signiﬁcantly elevated. There are no antibodies to MPO. This pattern is associated with Wegener’s granulomatosis. If the anti-neutrophil cytoplasmic antibodies are positive with either pattern but MPO and PR3
antibodies are negative (< 10 Enz Units), then the antibodies are probably against another target
antigen, and are of no known clinical signiﬁcance. If an antinuclear antibody is present there will be an appropriate comment in the report. Does ANCA have a role in the management of vasculitis Is ANCA a risk factor for a relapse of the vasculitis?
Relapse of vasculitis is frequent, and over a 5 year period, occurs in about 50% of Wegener’s
granulomatosis and 30% of microscopic polyangiitis. Relapse is more common in patients who have a
persistence of ANCA after clinical remission.This has been clearly demonstrated for PR3-ANCA, but
not for MPO-ANCA. Vasculitis with PR3-ANCA is associated with more frequent relapses than MPO-ANCA irrespective of
the diagnosis.This means that PR3-ANCA positive Wegener’s granulomatosis is more likely to relapse
than MPO-ANCA positive Wegener’s granulomatosis. Persistence of ANCA after remission of the vasculitis is also associated with a worse outcome. Patients
with MPO-ANCA associated glomerulonephtritis with persistent elevation of MPO-ANCA are more
likely to have end stage renal disease. Patients who initially are negative during remission and subsequently become positive are also at
increased risk of a relapse. Patients with a consistently negative ANCA after remission have a low probability of relapse. Therefore,
patients with persistence of ANCA, or the reappearance of ANCA should be more carefully monitored
for a ﬂare in their disease. How often should ANCA be measured?
Serial measurement of ANCA has been advocated as a means of predicting a disease relapse. However,
there is a poor correlation between titres and disease activity. Rising titres of PR3-ANCA may predict
relapses but there is no clear consensus as to what is a signiﬁcant rise. At present the best criteria are a 75
% increase in PR3-ANCA levels or a fourfold increase in IIF titre of C-ANCA. The frequency of testing has not been determined but it is considered that 6-8 weeks is reasonable.
However, the frequency of subsequent testing is likely to be determined by the severity of the vasculitis
Should the ANCA result be used to change treatment?
The role of pre-emptive treatment based on an increase in ANCA to prevent a clinical relapse is still a
controversial area. Two prospective studies have suggested that pretreatment with either azathioprine or
cyclophosphamide reduces relapses. There was a relapse rate of about 25% in those given pre-emptive
treatment compared to about a 60% relapse rate in those not given pre-emptive treatment. However, 25% to 40% of those given pre-emptive treatment would have received unnecessary
treatment. The beneﬁts from pre-emptive treatment should be related to the number of patients that
will have complications from an unnecessary increase in treatment. The persistence of ANCA suggests
that the vasculitis is not well controlled, and careful monitoring is required so that treatment can be
quickly altered if there are clinical features of a relapse.
What other diseases have a positive ANCA?
ANCA is present in numerous diseases other than systemic vasculitides. ANCA does not have any
diagnostic value in these situations, but their presence can cause uncertainty and result in an incorrect
diagnosis. It appears as though some of these ANCAs found on IIF are not true ANCAs and will
probably be reclassiﬁed. The following are some of these diseases associated with ANCA: Gastrointestinal Diseases
Several gastrointestinal diseases are associated with a positive ANCA, usually only on IIF. These include
ulcerative colitis where ANCA may be present in 60-80%, primary sclerosing cholangitis with a positive
ANCA in 60-80% and Crohn’s disease with a positive ANCA in 10-40%. The pattern is usually a
P-ANCA or atypical ANCA directed against a variety of target antigens. These include cathepsin G,
elastase, lysozome and lactoferrin and MPO. However, the most important antigens are located in the
chromatin and nuclear membrane and are not true ANCA. ANCA and Rheumatoid Arthritis and Systemic Lupus Erythematosus
ANCA is also found in a small percentage of patients with rheumatoid arthritis and systemic lupus
erythematosus. There is no association with the development of vasculitis in these diseases. ANCA and Chronic Bacterial Infections
Patients with chronic recurrent infections, particularly cystic ﬁbrosis and bronchiectasis may develop
ANCA. ANCA is present in about 90% of patients with cystic ﬁbrosis. This is usually a P-ANCA
pattern, with the target antigen being bacterial permeability-increasing protein. ANCA and Drug Induced Diseases Drug induced vasculitis or lupus erythematosus like syndrome may be associated with a positive ANCA.
The drugs most commonly producing ANCA are hydrallazine, minocycline and propylthiouracil. The commonest staining pattern is P-ANCA which is directed against several neutrophil derived
antigens. These include MPO, elastase, PR3 and cathepsin G. Antibodies to both PR3 and MPO may
develop together in drug induced ANCA. The combination of antibodies to both PR3 and MPO does
not occur in the ANCA associated vasculitides. When the drug is discontinued the clinical features and
• ANCA is a useful test only if there are clinical features suggestive of a vasculitis. • ANCA has no value as a general screening test for vasculitis. • Both the IIF and ELISA must be positive to support a diagnosis of vasculitis
• ANCA identiﬁes only a small number of vasculitides, the ANCA associated vasculitides. • ANCA may be present in other disorders but does not have any diagnostic or clinical relevance. • ANCA can be drug induced. • ANCA does not replace a biopsy of the involved tissue to make a diagnosis of vasculitis. • A rapid increase or reappearance of antibodies to PR3 or MPO, in patients with an ANCA
associated vasculitis should alert the physician to the possibility of a ﬂare in the vasculitis. Further reading
Schmitt WH, van der Woude FJ. Clinical applications of antineutrophil cytoplasmic antibody testing.
Curr Opin Rheumatol 2003;16:9-17. Sinclair D, Saas M, Stevens JM. The eﬀect of a symptom related “gating policy” on ANCA requests in
routine clinical practice. J Clin Pathol 2004;57:131-134. Tervaert JWC. ANCA testing in monitoring the activity of the disease. Kidney Blood Press Res
2003;26:226-230. Wiik A. Rational use of ANCA in the diagnosis of vasculitis (Editorial). Rheumatology 2002;41:481-483. INFOLINK FOLDERS AVAILABLE If you would like a folder to hold your copies of our Infolink newsletter, please email Adrienne Broe at firstname.lastname@example.org (limited stock)
Jornal Mulier – Agosto de 2006, Nº 31 Anorexia, depressão, histeria, síndrome de pânico: mulheres são as principais vítimas de transtornos psicossomáticos Um dos grandes enigmas da humanidade, os transtornos mentais até hoje procuram ser explicados por diversas áreas do conhecimento. Ao menos já se sabe que estas doenças têm raízes psicológicas, biológicas, sociais e cult
Name ______________________________________________ Date _____________________________ Date of last health care exam: ________________What was this exam for?_________________________ Have you been hospitalized in the last 5 years? (Please circle) If yes, reason:__________________________________________________________________________ Are you currently receiving care? No Yes If yes, nature of c