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Santa Cruz Biotechnology, Inc.
NKCC1 (N-16): sc-21545
BACKGROUND
Na-K-Cl cotransporters (NKCC) are channel proteins that aid in Store at 4° C, do not freeze; stable for one year from the date of the transcellular movement of chloride across both secretory and absorptive epithelia (1,2). The 170 kDa NKCC1 is expressed inmuscle cells, neurons, and red blood cells (1-3). In the basolateral RESEARCH USE
membrane of secretory epithelia, NKCC1 mediates active chloride For research use only, not for use in diagnostic procedures.
secretion (3). The gene encoding human NKCC1 maps tochromosome 5q23.3 (2). In mice, disruption of the NKCC1 gene REFERENCES
leads to deafness and impaired balance (4). NKCC2 is specificallyexpressed in the kidney where it mediates active reabsorption of 1. Xu, J.C., Lytle, C., Zhu, T.T., Payne, J.A., Benz, E., Jr., and sodium chloride in the thick ascending limb of the loop of Forbush, B., III 1994. Molecular cloning and functional expression Henle (3). NKCC2 is sensitive to the clinically important diuretics of the bumetanide-sensitive Na-K-Cl cotransporter. Proc. Natl.
furosemide and bumetanide (3). The gene encoding human NKCC2 maps to chromosome 15q15-q21 and mutations in this 2. Payne, J.A., Xu, J.C., Haas, M., Lytle, C.Y., Ward, D., and gene lead to Bartter’s syndrome, an inherited hypokalaemic Forbush, B., III 1995. Primary structure, functional expression, alkalosis (3,5). NCCT is a thiazide-sensitive Na-Cl cotransporter and chromosomal localization of the bumetanide-sensitive that is primarily expressed in the distal convoluted tubule of the Na-K-Cl cotransporter in human colon. J. Biol. Chem. kidney where it accounts for a significant fraction of net renal sodium reabsorption (5). The gene for human NCCT map tochromosome 16q13 (6). Mutations in the gene encoding NCCT 3. Quaggin, S.E., Payne, J.A., Forbush, B., III, and Igarashi, P.
cause Gitelman’s syndrome, a subset of Bartter’s syndrome (5,7).
1995. Localization of the renal Na-K-Cl cotransporter gene (Slc12a1) on mouse chromosome 2. Mamm. Genome NKCC1 (N-16) is an affinity purified goat polyclonal antibody 4. Delpire, E., Lu, J., England, R., Dull, C., and Thorne, T. 1999.
raised against a peptide mapping near the amino terminus of Deafness and imbalance associated with inactivation of the secretory Na-K-2Cl co-transporter. Nat. Genet. 22: 192-195.
5. Simon, D.B., Nelson-Williams, C., Bia, M.J., Ellison, D., Karet, F.E., Molina, A.M., Vaara, I., Iwata, F., Cushner, H.M., Each vial contains 200 µg IgG in 1.0 ml of PBS containing Koolen, M., Gainza, F.J., Gitleman, H.J., and Lifton, R.P. 1996.
Gitelman's variant of Bartter's syndrome, inherited hypokalaemicalkalosis, is caused by mutations in the thiazide-sensitive Na-Cl Blocking peptide is available for competition studies (sc-21545 P) cotransporter. Nat. Genet. 12: 24-30.
(100 µg peptide in 0.5 ml PBS with 0.1% sodium azide and 100 µg BSA). 6. Mastroianni, N., De Fusco, M., Zollo, M., Arrigo, G.,Zuffardi, O., Bettinelli, A., Ballabio, A., and Casari, G. 1996.
SPECIFICITY
Molecular cloning, expression pattern, and chromosomal NKCC1 (N-16) is recommended for the detection of NKCC1 of localization of the human Na-Cl thiazide-sensitive mouse, rat and human origin by Western blotting and ELISA.
cotransporter (SLC12A3). Genomics 35: 486-493.
Recommended dilution range for Western blot analysis: 7. Mastroianni, N., Bettinelli, A., Bianchetti, M., Colussi, G., 1:100–1:1000. Recommended starting dilution: 1:100.
De Fusco, M., Sereni, F., Ballabio, A., and Casari, G. 1996. Novelmolecular variants of the Na-Cl cotransporter gene are responsiblefor Gitelman syndrome. Am. J. Hum. Genet. 59: 1019-1026.
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April 2001

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