Journal of Pediatric Gastroenterology and Nutrition 31:490–497 November 2000 Lippincott Williams & Wilkins, Inc., Philadelphia
Medical Position Statement: The North American Society for Pediatric
Helicobacter pylori Infection in Children: Recommendations for
*Benjamin D. Gold, †Richard B. Colletti, ‡Myles Abbott, §Steven J. Czinn, Yoram Elitsur,
¶Eric Hassall, #Colin Macarthur, **John Snyder, and ††Philip M. Sherman
Division of *Pediatric Gastroenterology and Nutrition, Department of Pediatrics, Emory University School of Medicine,Atlanta, Georgia; †Pediatric Gastroenterology, University of Vermont, Fletcher Allen Health Center, Burlington, Vermont;‡Berkeley California; §Pediatric Gastroenterology, Rainbow Babies and Children’s Hospital, Cleveland, Ohio; Division ofGastroenterology, Marshall University School of Medicine, Huntington, West Virginia; ¶Division of Gastroenterology, BCChildren’s Hospital, Vancouver, British Columbia, Canada; Divisions of #General Pediatrics and ††Gastroenterology andNutrition, The Hospital for Sick Children, Toronto, Ontario, Canada; and **Pediatric Gastroenterology, University of CaliforniaSan Francisco, San Francisco, California, U.S.A.Helicobacter pylori infects at least 50% of the world’s
been infected for many years before clinical symptoms
human population (1). However, most individuals in-
appeared (6). The incidence of H. pylori infection in
fected with H. pylori do not experience symptoms or
industrialized countries is estimated to be approximately
have signs of recognizable disease. In most children, the
0.5% of the susceptible population per year. In contrast,
presence of H. pylori infection does not lead to clinically
there is a significantly higher estimated incidence of H.
apparent disease, even when the organism colonizing the
pylori infection in developing countries of approximately
gastric mucosa causes chronic active gastritis (2).
3% to 10% per year (7). The limited data on the inci-
Knowledge about H. pylori infection is evolving, par-
dence of H. pylori infection in children consist largely of
ticularly in the pediatric age group for which there are
retrospective seroprevalence studies.
Humans appear to be the primary natural reservoir of
Additional multicenter, randomized, placebo-
H. pylori infection. Other reservoirs that have been pro-
controlled treatment trials in children infected by H. py-
posed include water, domestic cats, and houseflies (8–
lori are critically needed to definitively characterize the
10). The risk factors described for acquiring infection
effect of H. pylori eradication treatment during child-
include residence in a developing country, poor socio-
hood on symptoms and gastroduodenal mucosal disease.
economic conditions, family overcrowding, and possibly
There is compelling evidence that this organism is
an ethnic or genetic predisposition. In North America,
associated with a significant proportion of duodenal ul-
the prevalence rates of H. pylori among Asian-Ameri-
cers and, to a lesser extent, with gastric ulcers in children
cans, African-Americans and Hispanics are similar to
(3). There are epidemiologic data linking chronic H. py-
those of residents of developing countries (11). The route
lori infection, probably beginning in childhood, with the
of transmission of H. pylori in humans is not known but
development of gastric adenocarcinoma and gastric lym-
is postulated to be fecal-oral, gastric-oral (in vomitus), or
phoma (4). Findings in recently reported animal models
support the role of H. pylori in the pathogenesis of gas-
Although H. pylori infection may be acquired during
childhood, there are limited guidelines regarding its di-
There are many studies describing the prevalence of
agnosis and treatment in children and adolescents. Such
H. pylori infection. Most epidemiologic studies of H.
evidence-based consensus guidelines are needed for both
pylori infection have been performed in adults who had
primary care and specialty medical providers to ensurejudicious use of diagnostic testing and appropriate thera-peutic regimens for the management of children with H.
Received and accepted September 8, 2000. pylori infection. Therefore, the North American Society
Address correspondence and reprint requests to Dr. Benjamin Gold,
for Pediatric Gastroenterology and Nutrition (NASPGN)
Emory University School of Medicine, 2040 Ridgewood Drive, NE,
appointed the Helicobacter pylori Infection Guideline
Atlanta, GA 30322, U.S.A.; or to Dr. Richard B. Colletti, University of
Committee to develop a clinical practice guideline for
Vermont, Department of Pediatrics, A-121 Given Medical Building,Burlington, VT 05405-5557, U.S.A.
the child with H. pylori infection. TREATMENT RECOMMENDATIONS FOR HELICOBACTER INFECTION
These clinical practice guidelines are designed to as-
TABLE 1. Summary of recommendations and the quality of
sist primary care physicians, nurse practitioners, physi-
cian assistants, and pediatric gastroenterologists in the
evaluation and treatment of suspected or diagnosed H.pylori–associated disease. The desired outcomes of these
How reliable are tests for H. pylori infection?
recommendations are the detection of children and ado-
Currently, the diagnosis of H. pylori-mediated disease
lescents with H. pylori who need treatment. These rec-
can be made reliably only through the use of
ommendations are applicable to children in developed
countries where the prevalence of infection is low but
Presently available commercial serologic tests are
may not be directly relevant to children living in com-
frequently unreliable for screening children for thepresence of H. pylori infection.
munities where there is a higher frequency of gastric
Urea breath testing, although promising, has not been
colonization by H. pylori. These recommendations have
been endorsed by the Executive Council of NASPGN
When is testing indicated?
and by the American Academy of Pediatrics. They are
It is recommended that testing be performed in
children with endoscopically diagnosed, or
general guidelines to assist medical care providers in the
radiographically definitive, duodenal or gastric ulcers.
diagnosis and treatment of H. pylori infection in chil-
It is recommended that children with recurrent
dren. They are not intended as a substitute for clinical
abdominal pain, in the absence of documented
judgment or as a protocol for the management of all
ulcer disease, not be tested for H. pylori infection.
Testing for H. pylori infection is not recommended in
In its deliberations, the committee addressed four is-
Routine screening of children with a family history of
sues about H. pylori infection in children: How reliable
gastric cancer or recurrent peptic ulcer disease is
are tests to detect H. pylori? When is testing for H. pylori
indicated? When is treatment of H. pylori infection in-
Testing following treatment of documented H. pylori
is recommended, especially with complicated
dicated? What is the preferred treatment of H. pylori? A
peptic ulcer disease (i.e., bleeding, perforation, or
summary of the recommendations of the H. pylori Infec-
obstruction). For patients who remain symptomatic
tion Guideline Committee is presented in Table 1.
after treatment, it is recommended that endoscopyand biopsy be performed to evaluate for thepersistence of H. pylori-associated peptic ulcer
If pathological evidence of MALT lymphoma is
The H. pylori Infection Guideline Committee consisted of a
documented, then testing for H. pylori is
primary care pediatrician, a clinical epidemiologist, and seven
pediatric gastroenterologists. To develop evidence-based
When is treatment of H. pylori infection indicated?
guidelines, articles published in English from January 1966
Eradication treatment is recommended for children who
through May 1999 on H. pylori in children were searched.
have a duodenal ulcer or gastric ulcer identified atendoscopy and H. pylori detected on histology.
Articles on diagnosis and treatment were sought separately.
A prior history of documented duodenal or gastric
Letters, editorials, case reports, abstracts, and reviews were
ulcer disease is an indication for treatment if active
excluded. Evidence tables were prepared based on 16 articles
H. pylori infection is documented.
on clinical presentation, 9 articles on diagnostic studies, and 30
There is no compelling evidence for treating
articles on therapy. Subsequently, additional articles were iden-
children with H. pylori infection and non-ulcer
tified and reviewed. When the pediatric literature was insuffi-
dyspepsia or functional recurrent abdominal pain.
cient, the adult literature was also considered. Articles were
Treatment is not recommended for H. pylori-infected
evaluated using published criteria (13). The Committee based
children residing in chronic care facilities; children
its recommendations on an integration of a review of the medi-
with unexplained short stature; or children atincreased risk for acquisition of infection, including
cal literature and expert opinion. Consensus was achieved by
asymptomatic children who have a family member
using the nominal group technique, a structured quantitative
with either peptic ulcer disease or gastric cancer.
method, as described previously (14,15). By using the methods
What is the preferred treatment of H. pylori infections in children?
of the Canadian Preventive Services Task Force (16), the qual-
It is recommended that treatment consist of three or
ity of evidence of each of the recommendations made by the
four medications, given once or twice daily, for
committee was determined and is summarized (Table 1). a Categories of the quallity of evidence: I Evidence obtained from at
HOW RELIABLE ARE TESTS FOR
least one properly designed randomized controlled study. II-1 Evidence
H. PYLORI INFECTION?
obtained from well-designed cohort or case-controlled trials withoutrandomization. II-2 Evidence obtained from well-designed cohort orcase-control analytic studies, preferably from more than one center or
Several invasive and noninvasive tests are available to
research group. II-3 Evidence obtained from multiple time series with
detect H. pylori infection (Table 2). An ideal test for H.
or without the intervention. Dramatic results in uncontrolled experi-
pylori is noninvasive or minimally invasive, highly ac-
ments (such as the results of the introduction of penicillin treatment in
curate, inexpensive, and readily available and enables
the 1940’s) could also be regarded as this type of evidence. III Opinionsof respected authorities, based on clinical experience, descriptive stud-
differentiation between active or past infection with the
ies, or reports of expert committees. J Pediatr Gastroenterol Nutr, Vol. 31, No. 5, November 2000TABLE 2. Tests for Helicobacter pylori and
study. The optimal staining of biopsy sections is best
determined by local expert pathologists. Endoscopic ex-amination of and specimens obtained in the esophagus,
stomach, and duodenum also provide information about
Biopsies and histologyRapid urease testing
other upper gastrointestinal disorders that may be the
cause of clinical symptoms including, for example,
Polymerase chain reaction of bacterial DNA
esophagitis and peptic ulcer disease that is not due to H.
Serum and whole blood antibodySaliva antibody
There are drawbacks to diagnostic gastrointestinal en-
doscopy. It is a relatively invasive procedure requiring
sedation or anesthesia. Furthermore, the test remains
relatively expensive in many centers, and access to anendoscopist with specific pediatric expertise is limited inmany geographic areas.
organism. In addition, such a test enables discriminationbetween the presence of H. pylori infection and H. py-lori–associated disease. Because no such ideal test cur-
Rapid Urease Testing of Biopsy Tissues
rently exists, the advantages and drawbacks of tests that
Urease testing (CLO, TriMed, Kansas City, MO; Hp-
are available require critical appraisal and must be as-
Fast, GI Supply, Division of ChekMed Systems Inc.,
sessed for their suitability for use in children.
Camphill, PA; PyloriTek, Horizons International, Agua-
Failure to reach an accurate diagnosis carries consid-
dilla, Puerto Rico) provides indirect identification of H.
erable financial and social costs including the expense of
pylori infection within a few hours of endoscopy (20).
more tests, repeated visits to health care providers, inap-
However, these tests have a poor positive predictive
propriate treatment, and missed school or work. A de-
value (as low as 50%) in children, even though the nega-
finitive test, even if it is expensive, may result in overall
tive predictive value is high (97–98%) (20,21). The ac-
curacy of the test is dependent on the number of tissue
It is important to emphasize that the accuracy of a
specimens tested, the location of biopsy sites, bacterial
diagnostic test is greatly impacted by the prevalence of
load, and previous usage of antibiotics and proton pump
H. pylori in the population tested. There is a need for
inhibitors, as well as the prevalence of H. pylori in the
studies to assess the accuracy and potential utility of
various noninvasive diagnostic tests in populations inNorth America that differ in demographic factors thatmay influence the prevalence and natural history of H.
Culture of H. pylori from the gastric mucosa provides
an opportunity to obtain a profile of antibiotic sensitivity
Invasive Testing Through Endoscopy
that could identify potential treatment failure due to an-tibiotic resistance (22). Culture also provides a bacterial
strain for use in epidemiologic studies to examine asso-ciations of virulence characteristics with disease out-
The definitive diagnosis of H. pylori and the evidence
come. However, bacterial culture for H. pylori is rela-
of the consequences of infection can be made reliably
tively expensive and success rates for recovery of the
only by endoscopy with multiple biopsy specimens ob-
organism in many clinical laboratories are low (23). Cur-
tained in one or more regions of the stomach including
rently, standardization of culture procedures has not been
antrum, body, and transition zones (i.e., cardia and inci-
established, and bacterial cultures are only obtained rou-
sura). Histology provides information regarding the pres-
ence of H. pylori and the severity and topographic dis-tribution of gastritis including the presence of atrophic
gastritis, intestinal metaplasia, and mucosa-associatedlymphoid tissue (MALT) lymphoma (3). As in adults,
Polymerase chain reaction (PCR) is a highly sensitive
biopsy specimens obtained in the prepyloric antrum have
technique that can be used to detect the presence of H.
the highest yield in H. pylori infection. Tissue specimens
pylori in body fluids (e.g., gastric juice and stool), tissues
often are also obtained from the body and the transition
(e.g., gastric mucosa), and water (24). Testing of H. py-
zones of the stomach, particularly if the patient has re-
lori genomic DNA by PCR can be used to advance
cently taken acid-suppressing medication (19). It is rec-
knowledge at the molecular level—for example, by pro-
ommended that multiple biopsies be performed in chil-
viding information about point mutations conferring
dren with endoscopically documented peptic ulcer dis-
resistance to antibiotics and about putative bacterial
ease or peptic ulcer suspected as a result of radiographic
virulence factors. However, PCR is expensive, the assay
J Pediatr Gastroenterol Nutr, Vol. 31, No. 5, November 2000TREATMENT RECOMMENDATIONS FOR HELICOBACTER INFECTION
is difficult to set up, specificity may be compromised by
cation therapy. However, patients may be reluctant to
inadvertent contamination, and it is not widely available
collect stool specimens. In addition, refrigerated stools
are more difficult to test. Additional pediatric studiesevaluating the accuracy of stool antigen testing for bothinitial diagnosis and posttreatment follow-up are re-
quired before specific recommendations can be consid-
Immunoassay Tests to Detect H. pylori Antibodies
Enzyme-linked immunosorbent assays (ELISAs) to
detect H. pylori antibodies are relatively inexpensive andeasy to implement in the clinical setting. Many tests areavailable for use to test whole blood, plasma, or serum.
Urea breath tests are noninvasive and have high sen-
However, compared with histology, the sensitivity and
sitivity and specificity (>95%) both in adults (33) and
specificity of serologic assays are poor in both adults and
children (34,35). The test requires the ingestion of either
children unless used in the populations in which they
radiolabeled 14C-urea or urea tagged with the stable iso-
were initially developed (25). In general, the accuracy of
tope 13C. Test results may be influenced by concurrent
serum-based immunoassays and whole-blood tests for
use of antibiotics and acid-suppressing medications and
use in the physician’s office in symptomatic children in
by the presence of other urease-producing organisms
developed countries is poor, with a range of sensitivity of
present in the oral cavity. Test parameters are currently
only 60% to 70% (26–28). Furthermore, age-related cut-
laboratory-specific (e.g., dosages for differing ages of
off values for commercial immunologic tests have not
children, cutoff values, duration of fasting, use of a test
been established for children. One immunoassay devel-
meal, times of sampling, and timing of posttherapy test-
oped in a research center to detect H. pylori–specific
ing) and have not been well standardized for children
immunoglobulin (Ig)G in children was 91% sensitive
(36). In addition, urea breath testing is technically more
compared with sensitivity of less than 70% in three com-
difficult to perform in small children and infants, with
mercially available assays (28). In areas with low preva-
failure rates in collection up to 10%, especially outside
lence of H. pylori infection, such as in developed coun-
tries, testing of serum and whole blood is not sufficiently
In summary, the diagnosis of H. pylori–associated dis-
accurate to diagnose H. pylori infection in children. Ac-
eases currently can be made reliably only by endoscopy
cordingly, treatment regimens based on the results of
with biopsies. The most commonly used noninvasive test
these tests cannot be recommended. Serologic tests may
to screen adults for H. pylori infection is serology. Un-
not be used reliably to verify eradication of H. pylori,
fortunately, currently available commercial serologic
because antibody titers can remain positive for months,
tests are frequently unreliable for screening children for
the presence of H. pylori infection. Current whole-blood,saliva, and urinary immunoassays are insufficiently sen-sitive or specific to be effective as diagnostic tools. In-
Saliva and Urine Tests for H. pylori Antibodies
sufficient data are available in children to confirm theaccuracy of the recently approved H. pylori stool antigen
Similar to serologic tests, saliva-based tests also detect
test. The urea breath test has the promise to provide
the presence of H. pylori–specific IgG antibodies. The
noninvasive and accurate diagnosis of H. pylori infec-
tests are easy to perform, painless, and inexpensive. Sa-
tion; but currently, there is insufficient evidence that it
liva tests are less sensitive than assays of serum or whole
can be used to reliably diagnose or exclude H. pylori–
blood (29). The protein concentration of saliva appears to
affect the accuracy of test results. Urine-based assays areeasy to perform, require minimal labor for collection,and are painless (30). However, these assays are highlyvariable and are not yet commercially available. There-
WHEN IS TESTING INDICATED?
fore, saliva and urine assays for the detection of H. pyloriantibodies cannot be recommended.
The primary goal of testing is to diagnose the cause of
clinical symptoms and not simply to detect the presence
Stool Test for H. pylori Antigens
of H. pylori infection. Testing is not helpful unless it willalter the management of the disease.
Testing of H. pylori antigens in stools has shown
A variety of invasive and noninvasive tests exist for
promising results in adults for the noninvasive diagnosis
the detection of H. pylori infection, but their degree of
of gastric infection using a commercially available kit
sensitivity and specificity vary, as do their suitability for
(31). Testing for H. pylori antigens in feces also appears
clinical use in children. Thus, there is potential for inap-
to be accurate for use in monitoring the success of eradi-
propriate testing or misuse of tests in children. J Pediatr Gastroenterol Nutr, Vol. 31, No. 5, November 2000Endoscopically Diagnosed or Radiographically
dicates that there is a link between gastric cancers (both
Definitive Peptic Ulcer
adenocarcinoma and lymphoma) and H. pylori infection. However, no studies have shown that H. pylori eradica-
The causal relationship between H. pylori infection
tion during childhood prevents subsequent development
and primary duodenal ulcers is compelling (37). There-
of gastric malignancies. Until evidence is available to
fore, it is recommended that testing for the presence of H.
better define the role of H. pylori in a variety of gastric
pylori infection be performed in children with endo-
cancers and the role of H. pylori eradication in disease
scopically diagnosed or radiographically definitive duo-
prevention, routine screening of children with a family
denal ulcer. Although the data in children are less com-
history of gastric cancer or recurrent peptic ulcer disease
plete, evidence from studies in adults (38) supports the
recommendation that testing for H. pylori also be per-formed in subjects with a documented gastric ulcer. Histologic Evidence of Lymphoma Abdominal Pain Unrelated to Peptic Ulcers
In the rare circumstance in which histopathologic evi-
Several lines of evidence, including serologic surveys,
dence of MALT lymphoma is documented in a child,
endoscopic evaluations, and treatment trials indicate that
testing for H. pylori is recommended. H. pylori is not a frequent cause of recurrent abdominalpain in children. There have been six studies performedin North America, Europe, and Australia, with 2715 chil-
Follow-up of Therapy for H. pylori Infection
dren evaluated by esophagogastroduodenoscopy and bi-opsy, serology, or urea breath test (39–44). Although 5%
Testing to confirm eradication of infection and the
to 17% of children with abdominal pain had evidence of
resolution of associated symptoms and disease sequelae
infection with H. pylori, 5% to 29% of children without
is advisable in selected children. Guidelines in adults
abdominal pain were also infected with H. pylori. There
recommend testing after treatment of complicated peptic
are no convincing data to support routine testing of chil-
ulcer (52), but studies in children are limited. As such,
dren with recurrent abdominal pain (39–45). Investiga-
few data are available on the effectiveness of therapy in
tors have also looked for specific symptom patterns in H.
children, testing after treatment is recommended in those
pylori–infected children, but none so far has been de-
with complicated peptic ulcer disease (i.e., bleeding, per-
tected (46–50). Future studies are needed to determine
foration, or obstruction) or lymphoma. For patients who
whether subsets of children with abdominal pain can be
remain symptomatic, it is recommended that endoscopy
identified in whom signs and symptoms are caused by H.
and biopsy be performed to evaluate for the persistence
pylori infection. It is recommended that children with
of H. pylori-associated peptic ulcer disease. For patients
recurrent abdominal pain, in the absence of documented
with an uncomplicated ulcer who are asymptomatic after
ulcer disease, not be tested for H. pylori infection.
completion of eradication therapy, testing for persistenceof infection is not necessary. However, some physicians
Asymptomatic Children, Including Those at
advocate the use of urea breath testing in this clinical
Increased Risk of Acquiring H. pylori Infection
There are no compelling data to support routine testing
in asymptomatic children. Testing for H. pylori infection
WHEN IS TREATMENT OF H. PYLORI
is not recommended in children without clinical symp-
toms, including those residing in long-term care facili-ties, children with short stature, and those at increased
Eradication therapy is recommended for children who
risk of acquiring H. pylori infection. In addition, pur-
have both known active H. pylori infection and symp-
ported extraintestinal manifestations of H. pylori infec-
tomatic gastrointestinal disease. Known active H. pylori
tion have not been demonstrated in a convincing fashion
infection is defined as identification of the organisms by
(51). Accordingly, a test-and-treat approach is not rec-
histopathologic examination or as a positive culture from
endoscopic gastric biopsy. Serology is not a reliable testfor active disease, because it may indicate past but not
Family History of Gastric Cancer or Recurrent
current infection with H. pylori. Peptic Ulcer Disease
There are no randomized controlled trials in children
that determine the precise clinical settings in which
No currently available data support routine testing in
eradication therapy is indicated. Although additional
children with a positive family history of diseases related
studies in children are needed (53), the available evi-
to H. pylori infection (52). Epidemiologic evidence in-
dence supports the following recommendations. J Pediatr Gastroenterol Nutr, Vol. 31, No. 5, November 2000TREATMENT RECOMMENDATIONS FOR HELICOBACTER INFECTIONDuodenal and Gastric Ulcers
cost of care. Therefore, the H. pylori Infection GuidelineCommittee concludes that there is insufficient evidence
Eradication treatment is recommended for children
to support either initiating or withholding eradication
who have a duodenal ulcer or gastric ulcer identified at
endoscopy and H. pylori documented by histopathology. A prior history of duodenal or gastric ulcer disease is alsoan indication for treatment if active H. pylori infection is
Recurrent Abdominal Pain and
documented. If a definitive ulcer is present on contrast
radiography (e.g., an ulcer crater is present), eradicationtherapy is indicated if either a noninvasive or invasivetest result is positive for H. pylori.
There is no compelling evidence, at the present time,
for treating children with H. pylori infection and either
nonulcer dyspepsia or functional recurrent abdominalpain. There is also no convincing evidence currently
The rare child with pathologic evidence of MALT
available that asymptomatic children who have a family
lymphoma and H. pylori infection should be treated with
member with H. pylori infection, peptic ulcer, or gastric
eradication therapy. Further studies of pediatric patients
with lymphoma should be performed to monitor the re-currence, progression, or remission of the tumor aftertherapy. WHAT IS THE PREFERRED TREATMENT OF H. PYLORI INFECTION IN CHILDREN? Atrophic Gastritis With Intestinal Metaplasia
The optimum treatment regimen for eradicating H. py-
Eradication treatment is recommended for the rare
lori in children has not been determined (59). Effective
child who has pathologically proven atrophic gastritis
therapy in adults is defined as successful eradication of
with intestinal metaplasia, according to the updated Syd-
H. pylori infection in a minimum of 80% of treated sub-
ney classification of gastritis (54), plus coexisting H.
jects (60). Although it appears that treatment options that
pylori infection. Because of the preneoplastic nature of
have been effective in adults will also be efficacious in
these pathologic changes, follow-up endoscopy is rec-
children, controlled studies in pediatric populations are
ommended to confirm that the H. pylori infection has
needed to confirm or refute this supposition. Unfortu-
been eradicated and to ensure that there is no subsequent
nately, the limited data currently available in children are
progression of gastric mucosal disease.
open-label, case series and uncontrolled, anecdotal ob-servations that do not meet the minimum criteria for
Gastritis Without Peptic Ulcer Disease
determining efficacy. In vitro sensitivity of H. pylori to aspecific drug does not guarantee that the bacterium will
The finding of H. pylori–associated gastritis in the
be effectively eradicated from the human stomach.
absence of peptic ulcer disease during diagnostic endos-
Therefore, current treatment strategies to eradicate H.
copy poses a dilemma for the endoscopist. The decision
pylori have been developed primarily by trial-and-error
to treat H. pylori–associated gastritis without duodenal or
gastric ulcer in this situation is subject to the judgment of
The single most important determinant of successful
the clinician and deliberations with the patient and fam-
eradication therapy is compliance with the prescribed
ily. Studies in adults on the effect of eradication treat-
combination treatment regimen (62). There are well-
ment on abdominal symptoms have produced conflicting
described treatment failures due to suboptimal compli-
results (55–58). There are no randomized controlled tri-
ance. To enhance adherence to the treatment regimen,
als in children. The long-term impact of the eradication
the number of medications prescribed, the frequency of
of H. pylori and the healing of gastritis on the subsequent
administration, and the duration of therapy are best kept
development of peptic ulcer disease, adenocarcinoma, or
to the minimum required for successful treatment.
lymphoma is uncertain. Although there is a small life-
It is recommended that initial treatment consist of
time risk of development of peptic ulcer disease associ-
three medications, administered twice daily, for 1 to 2
ated with H. pylori gastritis, there are no randomized
weeks (63). Specifically, as shown in Table 3, three first-
controlled trials demonstrating that eradication of H. py-
line therapy options are recommended for use in children
lori results in prevention of peptic ulcer disease. In ad-
and adolescents. For patients in whom initial treatment
dition, there are no data showing that eradication therapy
has failed, two other options are recommended, includ-
influences the long-term risk for development of gastric
ing one option with four medications. It is recommended
cancers. Antibiotic treatment can result in adverse drug
that monotherapy and two-drug regimens be avoided,
reactions, promote antibiotic resistance, and increase the
because they are ineffective and increase the likelihood
J Pediatr Gastroenterol Nutr, Vol. 31, No. 5, November 2000TABLE 3. Recommended eradication therapies for H. pylori
pump inhibitor also reduces the effectiveness of eradica-
tion treatment protocols. Studies are needed to determinethe relative importance of these risk factors in pediatric
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Detection of Chiral Compounds Using HPLC with CD Detection Chiral detectors provide valuable information since Experimental they only respond to optically active compounds. This Compounds were run on a Jasco HPLC equipped with allows the researcher to differentiate enantiomers. A a CD and an ORD detector. Sensitivity comparisons sensitivity comparison between the two chiral detecti