Site brasileiro onde você pode comprar qualidade e entrega viagra preço cialis barato em todo o mundo.
Developments in Plant Pathology
K. Rudolph, T.J. Burr, J.W. Mansfield,
D. Stead, A. Vivian and J. von Kietzell
Kluwer Academic Publishers
BASHAN,YOAV Department of Microbiology, The Center for Biological Research of the Northwest (CIB), A.P. 128, La Paz, 23000, B.C.S., Mexico
ALTERNATIVE STRATEGIES FOR CONTROLLING PLANT DISEASES
CAUSED BY PSEUDOMONAS SYRINGAE
The most common strategy for controlling diseases caused by Pseudomonas
is, as it has been for more than 5 decades, to spray bactericides. These mainly include a variety of copper compounds or other heavy metals, with or without various combinations of fungicides or other pest-control chemicals. Spraying with antibiotics or other organic bactericides have also been used, but to a lesser extent. Unfortunately, these strategies have never been satisfactory, resulting in heavy crop losses during severe epidemics. Over the last years, pathogenic strains that are resistant to copper spraying have been detected globally and are threatening the continuation of this strategy. Three different strategies are slowly being introduced into the field, (i) seed disinfection by heat or a combination of heat and bactericides, (ii) biological control by antagonistic microorganisms, and (iii) soil solarization especially against pathogens that spend part of their life cycle in the soil. Additional minor strategies include: (i) spraying infested plants with natural-occuring antibacterial compounds, and (ii) various techniques of sustainable agriculture without the use of chemicals (organic farming).
As long as the global agricultural production of fruits and vegetables
increases annually and uses current agro-technical procedures, the need for controlling pathovars of Pseudomonas syringae
major agricultural issue since these bacterial pathogens are among the most destructive known. For many bacterial diseases, current management techniques are often ineffective. Phytopathogenic bacteria can be symptomless for prolonged periods (8). Slight changes in the environment which favor the bacteria may cause a rapid outbreak, creating severe epidemics that can destroy the crop. The available means of controlling field epidemics are few, decades-old, rarely effective and too expensive for low value crops. Thus, the major approach is to prevent the development of epidemics, e.g. contain the pathogen when its level is low.
Attempts to control outbreaks and epidemics caused by Pseudomonas
pathovars have evolved little over the last century. Unfortunately, most of our knowledge on the control of bacterial disease is in the "gray zone" of science, being the domain of pesticide companies and popular agricultural magazines rather than peer-reviewed scientific journals. This makes it difficult to evaluate the field reliability of each strategy. Nevertheless this mini-review will try to sum up the major, minor, current, and future options for the researcher today, and hopefully, for the farmer in the future.
576 MAJOR CONTROL STRATEGIES
(i) Chemical control
The most common strategy for controlling diseases caused by Pseudomonas
pathovars is, as it has been for more than a century, to spray bactericides. These mainly include a variety of copper compounds (like the traditional "Bordeaux mixture", cupric hydroxide, copper sulfate, ammoniacal copper and copper salts of fatty acids) (21,46-48,64,67) or other heavy metals, with or without various combinations of fungicides or other pest-control chemicals. These compounds were the first biocides used for disease control and are the only bactericides registered and allowed for use on most crops. They were, and sometimes are, especially effective if applied in the proper manner (good sprayers, precise spraying schedules, good coverage of both sides of the leaf etc.) and with the proper timing (mainly prophylactic) (30,31). These factors are crucial to avoiding a wide-spread epidemic, a disease form which can hardly be controlled. Since the incidence of diseases caused by some P
pathovars is correlated with the epiphytic population of this species on plants before infection (28,45), such sprays are more effective if the pathogen has epiphytic survival ability since they can delay the establishment of high epiphytic pathogenic populations required for an epidemic (43,55,66). Spraying with antibiotics such as streptomycin or tetracycline alone, or combined with copper or other organic bactericides (12), is used (15) on a smaller field scale and mainly in greenhouse applications. Antibiotic treatments had limited success in tomatoes (31), but greater success in controlling pear blossom blast (11) and bacterial blight of coffee (32). The major difficulties of using antibiotics for agriculture are that they are difficult to register, or even prohibited (like in Australia) because of human health concerns (66) and the potential for disease resistance as occurred long ago in Xanthomonas campestris
Unfortunately, and despite of its wide usage, the chemical approach has not
been very satisfactory in the last decade. It provides protection only when the environmental conditions for disease development are limiting and results in heavy crop losses during severe epidemics (32). Most spray schedules almost never completely controlled any disease caused by P
pathovars. Increasing copper dosages are undesirable in many crops because of phytotoxicity (32,33,51), especially since dosages are already in their upper limits for most crops.
The most crucial factor against this strategy is the development of bactericide
resistance by the pathogens (60). Although they probably evolved undetected over the last 30 years , pathogenic strains that are resistant to copper spraying have been detected globally over the last decade (5,17,18,50). Development of bactericides resistance in bacterial communities does not require the independent evolution of resistance by each strain or species, but, rather, the community can evolve cooperatively by exchanging genetic information between strains, pathovars, species and genera (17,18). The way that cells of P. syringae
protect themselves against copper toxicity is probably by accumulating copper outside of the cells with periplasmatic copper biding proteins (18,19). Currently, copper-tolerant strains are claimed to be poorly controlled in the field by standard applications of copper compounds and therefore, threaten the future of the chemical strategies.
(ii) Seed disinfection by heat or a combination of heat and bactericides
In short season crops such as vegetables, an epidemic in the field might be
irreversible since even if the disease is reduced by other means later, it will not give the plant sufficient time to recover and compensate for the lost yield. Thus, seed disinfection might be a good preventive alternative to foliage spraying. Surprisingly, and despite plant protection laws of many countries (like Israel ) requiring seed disinfection or at least the sale of pathogen-free seeds, what little has been published on
pathovars in seeds is decades old (6,13,56,62).
Furthermore, many seed lots are regularly contaminated by bacterial pathogens despite the regulations, e.g., most of the commercial seed lots in Israel (imported or produced locally) were contaminated with one or more phytopathogenic bacteria (40). Common seed treatments are not always reliable since they appear to eliminate only bacteria that reside on the seed surface, but not bacteria inside the seeds. Even when their survival number is meager, the latter can survive for prolonged periods, are able to multiply under favorable environmental conditions and cause disease outbreaks in the field which may develop into epidemics later in the season (9,28,57). Therefore, an effective seed disinfection method must eradicate these few endophytic bacteria as well. Treatment of seed-borne P
with streptomycin that eliminates the pathogen (52) can not be considered applicable since such antibiotic treatment is not allowed for use in many countries as explained earlier in this review. Seed disinfection is done usually by heat treatments (thermotherapy). In essence, the seeds are suspended in hot water for various periods of time according to the tolerance of the pathogen and the tolerance of the seed to high temperatures. This approach represents an easy and preventive way of eradicating of bacterial pathogens, yet thermotherapy of highly infected seed lots is usually ineffective, especially with dry heat (27). Furthermore, these treatments are somewhat tricky since the long exposures to the high temperatures required to eradicate the pathogen are very close to what might damage the seed embryo (27). Thus, seed manufacturers tend to reduce the disinfection treatments, thereby reducing their effectiveness.
Alternative methods to toxic chemicals or high temperature disinfection
methods have been developed. Aqueous suspensions of non-human toxic chemicals (calcium propionate, tartaric acid) or acidic cupric acetate at only 50oC controlled P
heat is probably essential to the procedure since it increases solvent uptake (42). A combination of several chemicals and warm temperatures (25-45oC) which form an organic copper complex in the seeds bathing solution, almost completely eradicated a broad spectrum of tomato bacterial pathogens including P
. Most of the tomato seeds produced or imported by Israel are now treated using this method (41).
In sum, there is no apparent general method for eradicating bacterial
pathogens from seeds. Each pathogen and plant species might need modification of the methods described above. Currently, it appears that a combination of temperature and inhibitory chemicals are most effective under realistic seed manufacturing and plant growth conditions.
(iii) Biological control by antagonistic microorganisms
Biological control by antagonistic microorganisms is a well developed
concept, mainly for soil -borne pathogens and almost exclusively against fungal diseases (38). The first commercial products of this approach are already in the market, but only on a small scale (7,61). Foliar biological control (apart from prevention of ice nucleation, ) receives minimal attention today, and very few studies relate to pathovars of P
(4,53). For example, in the last conference of Plant Growth-promoting Rhizobacteria of 1994 in Australia, most of the papers concerned biocontrol agents, yet none out of over 80 studies presented referred to foliar biocontrol. Nevertheless, since this management approach is gaining considerable momentum against soil-borne pathogens, its potential should be pointed out. Two general approaches may be the key for developing biocontrol foliar agents; (i) searching for natural microorganisms that have antagonistic characteristics to the pathogen, and (ii) increasing the efficiency of a strain through a better formulation or genetic manipulation (39). A few examples of each approach can be found in the literature.
A strain of P. putida
isolated from pepper fruits was able to inhibit a wide
spectrum of pathogens including 5 pathovars of P
in in vitro
studies. However, its field potential was tested only against Erwinia carotovora
subsp. carotovora on potatoes (44). A nonpathogenic, copper resistant Tn5 mutant of P
when co-inoculated with a pathogenic strain significantly reduced the incidence of bacterial speck of tomato in the greenhouse. But, when inoculated with a copper sensitive pathogen on plants treated with copper, the control was even better (16). Saprophytic P
provided complete protection against gray mold caused by Botrytis cinerea in wounded pear fruits (29).
The potential of foliar biological control of P
can be better evaluated
from studies on the control of other bacterial pathogens. Crown gall caused by Agrobacterium tumefaciens
has been successfully controlled on a commercial scale for over 15 years by the use of A. radiobacter
(producing mainly a specific antibiotic, "agrocin 84", and less by the antibiotic "agrocin 434" against agrobacteria). Unfortunately, Agrocin-84 is coded on a plasmid which can be transferred to pathogenic agrobacteria, a fact which makes the latter resistant to biocontrol by A
a mutant was constructed which no longer can transfer the agrocin plasmid to pathogenic agrobacteria retaining the biocontrol capacity (23,49,54).
At the moment, we have to accept the fact that foliar biocontrol is a long term
prospect for the development of a comprehensive biocontrol strategy for P
(iv) Soil solarization, especially against pathogens that spend part of their
life cycle in the soil.
Soil solarization is a non-chemical method for soil disinfection which captures
solar irradiation under a clear plastic mulch (26,35). Soil solarization was invented in Israel (36) where the agro-technical and climatic conditions are optimal for such a technology. In principle, soil solarization affects pathogens by heating wet soil to temperatures up to 50oC in the upper layer and to about 40oC at 20 cm deep (depending on the geographical area and soil type). The eradication of pathogens is achieved by the prolonged exposure to these temperatures (a few weeks to a few months (34). Excellent eradication of numerous fungal soil-borne plant pathogens
and subsequent yield enhancement was achieved during the years that this method was applied in Israeli fields. Nonetheless, it is not widely used there because it is highly crop-dependent and is inversely correlated with the availability of other soil disinfection methods available to the farmer (26). For unestablished reasons, soil solarization in Israel is considered less reliable than common fumigants and a slightly risky procedure, especially for cash crops. However, soil solarization is the only practical solution for soil disinfection when chemical fumigants (like methyl bromide) are phytotoxic to the next crop like onions or in organic farming where chemicals are banned. Soil solarization is more popular in crops that require no additional expense such as some winter vegetables which already grow in mulched soil. The only difference for the farmer between common commercial growth and solarized growth is the longer period of soil coverage, starting in mid-summer and leaving the mulch in its place until the end of the growing season in the next spring. This method is also accepted for container cultivation where the high organic matter content of the growing substrate prevents efficient disinfection by fumigants. Despite its efficiency in various areas of plant pathology (increasing the yield in 50-100%),
soil solarization is currently used to control soil fungal diseases (like Fusarium
, Rhizoctonia, Sclerotium rolfsii
) and nematodes (24,25,37).
To the best of my knowledge, soil solarization has not been methodically
tested as an effective method to control any foliar bacterial pathogen, despite the verbal statements of Israeli Agricultural Extension Service personal who claim that no severe incidence of bacterial epidemics have occurred in solarized soil. However, it is known for example, that P
can survive in the soil and infested soil can inoculate successfully tomato plants (10). This bacterium is very susceptible to medium temperatures (around 50ºC) (20) which soil solarization provides. Thus theoretically, soil solarization should control this pathogen. However, as stated by Greenstein (26) regarding winter cash crops like tomatoes, farmers prefer proven fumigant disinfection of the soil rather than novel methods such as solarization. In his 15 years-old review, Katan (34) pointed out the need to study soil solarization in relation to phytopathogenic bacteria. This need is as important today as it was 15 years ago.
MINOR CONTROL STRATEGIES FOR THE FAR FUTURE
None of the following methods ever passed the experimental stage for the
control of bacterial pathogens. They are suggested in this mini -review as
theoretical ideas for future evaluation with respect to P
(i) Spraying infested plants with natural-occurring antibacterial compounds
The antibacterial activity of tea and coffee wastes against P
and P. s
. pv. pisi
was evaluated in laboratory and greenhouse trials. It
was suggested that different components in these wastes may control these two
pathogens (2). (ii) Various techniques of sustainable agriculture without the use of chemicals
In organic farming magazines, there are many statements that the incidence
of bacterial disease, in general, in organic farming is much lower compared to large scale commercial agriculture. No scientific explanation for this has been provided.
580 It can be only proposed that these claims should be subjected to rigorous testing under accepted plant pathology procedures.
Several agro-technical practices are reported to reduce the incidence of
bacterial diseases in general, especially in semi-arid lands under drip irrigation, (14). It is well known that many pathovars of P
require free-water on the leaves for multiplication (28). Thus, methods which keep the leaves dry should be advantageous. However, I found meager information on this aspect in the scientific literature.
Common decontamination procedures like the burning of crop residues
together with tillage eliminate many bacterial pathogens, including P
. These treatments were even more efficient than spraying with the common copper substances or antibiotics (Donegan et al. 1992).
(iii) Induced systemic resistance
Induced systemic resistance is the phenomenon of activating the defense
mechanisms of the plants prior to disease development, either by environmental factors, by mic roorganisms or by both. Systemic resistance can be induced by pathogens, non-pathogens, seed treatments with Plant Growth-Promoting Rhizobacteria (PGPR) and microbial metabolites (63). Most studies were carried out against fungal pathogens. However, few studies on pathovars of P
showed that this approach may have potential in the future control of these diseases. Inoculation with two PGPR strains protected cucumber plants against P
. pv. lachrymans
(Liu, unpublished cited in 63) and inoculation with P. fluorescens
induced protection against P. s.
CONCLUSIONS AND FUTURE PROSPECTS
The control of pathovars of P
is one of the most neglected research
areas of phytobacteriology. Unfortunately, it has attracted little attention despite the heavy crop losses that these pathogens are able to induce. Ironically, the most common strategy, both in use and in registration, is the least efficient of all, chemical spraying. Resistant mutants which are appearing in the fields in alarming numbers will someday render this approach useless, leaving growers with no efficient means of protecting their crops. More advanced methods such as biological control, soil solarization and induced systemic resistance are in their infancy. It is my opinion that it will be difficult or perhaps impossible to control diseases caused by P
in the future. Clearly more research effort should be done in this field.
This paper is written in memory of the late Mr. Avner Bashan
from Israel who encouraged agricultural research. I want to thank Mrs. Hanna
Levanony, The Weizmann Institute of Science, Israel and Miss Neta Bashan for
helping in collecting of papers discussed in this review and to Dr. Roy Bowers for
constructive English corrections. This paper was supported by a travel grant from
the organizers of the conference.
1. Alstroem, S. 1991. Induction of disease resistance in common bean susceptible to halo blight
bacterial pathogen after seed bacterization with rhizosphere pseudomonads. J. Gen. Appl. Microbiol. 37, 495-501.
2. Alstroem, S. 1992. Antibacterial activity of tea and coffee wastes against some plant pathogenic
strains. J. Phytopathol. 136, 329-334.
3. Alstroem, S. 1994. A study on disease resistance induced by rhizosphere pseudomonad against
. In: Improving plant productivity with rhizosphere bacteria. (Eds.) M.H. Ryder, P.M. Stephens and G.D. Bowen. P. 148. CSIRO Division of Soils, Australia.
4. Amijee, F., Allan, E.J., Waterhouse, R.N., Glover, L.A., and Paton, A.M. 1992. Non-pathogenic
association of L-form bacteria (Pseudomonas syringae
) with bean plants (Phaseolus vulgaris
L.) and its potential for blocontrol of halo blight disease. Biocontrol Sci. Technol. 2, 203-214.
5. Andersen, G.L., Menkissoglou, O., and Lindow, S.E. 1991. Occurrence and properties of
coppertolerant strains of Pseudomonas syringae
isolated from fruit trees in California. Phytopathology. 81, 648-656
6. Baker, K.F. 1962. Thermotherapy of planting material. Phytopathology 52,1244-1255. 7. Backman, P.A., Brannen, P.M. and Mahaffee, W.F. 1994. Plant response and disease control
following seed inoculation with Bacillus subtilis
. In: Improving plant productivity with rhizosphere bacteria. (Eds.) M.H. Ryder, P.M. Stephens and G.D. Bowen. pp 3-8. CSIRO Division of Soils, Australia.
8. Bashan, Y., Azaizeh, M., Diab, S., Yunis, H. and Okon, Y. 1985. Crop loss of pepper plants
artificially infected with Xanthomonas campestris
in relation to symptom expression. Crop Protec. 4, 77-84.
9. Bashan, Y., Sharon, E., Okon, Y. and Henis, Y. 1981. Scanning electron and light microscopy of
infection and symptom development in tomato leaves infected with Pseudomonas tomato
. Physiol. Plant Pathol. 19, 139-144.
10. Bashan, Y. and Okon, Y. 1981. Inhibition of seed germination and development of tomato plants
in soil infested with Pseudomonas tomato
. Ann. Appl. Biol. 98, 413-417.
11. Bethell,R.S., Ogawa, J.M., English, W.H., Hansen, R.R., Manji, B.T., and Schick, F. J. 1977.
Copper-streptomycin sprays control pear blossom blast [Pseudomonas syringae
]. Calif. Agric. 31 (6): 7-9.
12. Brisset, M.N., Luisetti, J., and Gaignard, J.L. 1991. Firestop: a chemical against bacterial
diseases of fuit trees recently available in Europe [slow release formulation of flumequinine]. Agronomie 11, 93-99.
13. Chambers, S.C., and Merriman, P.R. 1975. Perennation and control of Pseudomonas tomato
Victoria. Aust. J. Agric. Res. 26,657-663.
14. Colin, J., Gerard, M., and Laabari, H. 1984. Influence du type d'irrigation sur la moucheture
bacterienne chez la tomate au Maroc. Parasitica 40:3 -12.
15. Conlin, K.C., and McCarter, S.M. 1983. Effectiveness of selected chemicals in inhibiting
in vitro and in controlling bacterial speck. Plant Dis. 67, 639-644
16. Cooksey, D.A. 1988. Reduction of infection by Pseudomonas syringae
nonpathogenic, copper-resistant strain combined with a copper bactericide. Phytopathology. 78, 601-603.
17. Cooksey, D.A. 1990. Plasmid-determined copper resistance in Pseudomonas syringae
impatiens. Appl. Environ. Microbiol. 56, 13-16
18. Cooksey, D.A. 1990. Genetics of bactericide resistance in plant pathogenic bacteria. Annu.
19. Cooksey, D., and Azad, H.R. 1992. Accumulation of copper and other metals by
copper-resistant plant-pathogenic and saprophytic pseudomonads. Appl. Environ. Microbiol. 58, 274-278.
20. Devash, Y., Okon, Y. and Henis, Y. 1980. Survival of Pseudomonas tomato
in soil and seeds.
21. Dillard, H.R. 1986. Control of fungal and bacterial diseases of processing tomatoes with foliar
sprays. Fungic. Nematic. Test Result 41, 64.
22. Donegan, K., Fieland, V., Fowles, N., Ganlo, L., and Seidler, R. 1992. Efficacy of burning,
tillage and biocides in controlling bacteria released at field sites and, effects on indigenous bacteria and fungi. Appl. Environ. Microbiol. 58, 1207-1214.
23. Fajardo, N.N., Tate, M.E., and Clare, B.G. 1994. Agrocin 434: an additional biological control
component for crown gall. In: Improving plant productivity with rhizosphere bacteria, Eds. Ryder, M.H., Stephens, P.M. and Bowen, G.D. CSIRO Division of Soils Australia. pp. 128-130.
24. Gamliel, A., and Katan, J. 1992. Influence of seed and root exudates on fluorescent
pseudomonads and fungi in solarized soil. Phytopathology 82, 320-327.
25. Gamliel, A. and Katan, J. 1993. Suppression of major and minor pathogens by fluorescent
pseudomonads in solarized and nonsolarized soils. Phytopathology 83, 68-75.
26. Grinstein, A. 1992. Introduction of a new agricultural technology -soil solarization - in Israel.
Phytoparasitica 20, (supplement) 1278-1318
27. Grondeau, C., Fourmond, A., Ladonne, F., Poulier, F., and Samson, R. 1992. Attempt to
eradicate Pseudomonas syringae
from pea seeds with heat treatments. Seed Sci. Technol. 20, 515-525.
28. Henis, Y. and Bashan, Y. 1986. Epiphytic survival of bacterial leaf pathogens. In: Microbiology of
the phyllosphere. (Eds.) N.J. Fokkema and J. van den Heuvel. Cambridge University Press, pp. 252-268.
29. Janisiewicz, W.J., and Marchi, A. 1992. Control of storage rots on various pear cultivars with a
saprophytic strain of Pseudomonas syringae
Plant Dis. 76, 555-560.
30. Jardine, D.J., and Stephens, C.T. 1987. A predictive system for timing chemical applications to
control Pseudomonas syringae
, causal agent of bacterial speck. Phytopatholog 77,823-827.
31. Jardine, D.J. and Stephens, C.T. 1987. Influence of timing of application and chemical on control
of bacterial speck of tomato. Plant Dis. 71, 405-408.
32. Kairu, G.M., Nyangena, C.M.S., and Crosse, J.E. 1985. The effect of copper sprays on bacterial
blight and coffee berry disease in Kenya. Plant Pathol. 34, 207-213.
33. Kairu, G.M., Nyangena, C.M.S. and Muthamia, J.E. 1991. The performance of copper-based
bactericides in the control of bacterial blight of coffee and coffee berry disease in Kenya. Trop. Pest Manage. 37, 1-5.
34. Katan, J. 1981. Solar heating (solarization) of soil for control of soilborne pests. Annu. Rev.
35. Katan, J. 1992. Soil solarization research as a model for the development of new methods of
disease control. Phytoparasitica 20, (supplement) 1338-1358.
36. Katan, J., Greenberger, A., Alon, H. and Grinstein, A. 1976. Solar heating by polyethylene
mulching for the control of diseases caused by soilbome pathogens. Phytopathology 66,683688.
37. Katan, J., Fishler, G., Grinstein, A. 1983. Short- and long-term effects of soil solarization and
crop sequence on Fusarium wilt and yield of cotton in Israel. Phytopathology 73, 1215-1219.
38. Kloepper, J.W. 1993. Plant growth-promoting rhizobacteria as biological control agents. In Soil
Microbial Technologies. (Ed). B. Metting, pp.255-274. Marcel Dekker, New York, NY.
39. Knudsen, G.R., Spurn H.W. Jr. 1988. Management of bacterial populations for foliar disease
biocontrol. In: Biocontrol of plant diseases, (editors) K.G. Mukerji, and K.L. Garg. CRC Press, Boca Raton, v. 1, p. 83-92.
40. Kritzman, G. 1991. A method for detection of seedbome bacterial diseases in tomato seeds.
41. Kritzman, G. 1993. A chemi-thermal treatment for control of seedborne bacterial pathogens of
42. Leben, C. 1983. Chemicals plus heat as seed treatments for control of angular leaf spot of
cucumber seedlings. Plant Dis. 67, 991-993
43. Legard, D.E. and Schwartz, H.F. 1987. Sources and management of Pseudomonas syringae
phaseolicola and Pseudomonas syringae
epiphytes on dry beans in Colorado. Phytopathology 77, 1503-1509.
44. Liao, C. H. 1989. Antagonism of Pseudomonas putide
strain PP22 to phytopathogenic bacteria
and its potential use as a biocontrol agent. Plant Dis. 73, 223-226.
45. Lindermann, J., Amy, D.C. and Upper, C.D. 1984.
Use of an apparent infection threshold
population of Pseudomonas syringae
to predict incidence and severity of brown spot of bean. Phytopathology 74,1334-1339.
46. MacNab, A.A. 1982. Effect of tank-mix holding time on tomato bacterial speck and early blight
control. Fungic. Nematic. Test Result 37, 84.
47. MacNab, A.A. 1982. Fungicide evaluation for early blight and bacterial speck control on
tomatoes. Fungic. Nematic. Test Result 3 7, 84.
48. MacNab, A.A. 1982. Tomato bacterial speck and early blight control with three rates of fixed
copper tank-mixed with Mancozeb. Fungic. Nematic. Test Result 37, 85.
49. McClure, N.C., Ahmadi, A.R., and Clare B.G. 1994. The role of agrocin 434 produced by
strain K84 and derivatives in the biological control of Agrobacteriun
biovar 2 pathogens. In: Improving plant productivity with rhizosphere bacteria, Eds. Ryder, M.H., Stephens, P.M. and Bowen, G.D. CSIRO Division of Soils Australia. pp. 125-127.
50. Menkissoglu, O. and Lindow, S.E. 1991. Chemical forms of copper on leaves in relation to the
bactericidal activity of cupric hydroxide deposits on plants. Phytopathology 81, 1263-1270.
51. Olson, B.D., and Jones, A.L. 1983. Reduction of Pseudomonas syringae
Montmorency sour cherry with copper and dynamics of the copper residues. Phytopathology 73, 1520-1525.
52. Pyke, N.B., Milne, K.S. and Neilson, H.F. 1984. Tomato seed treatments for the control of
bacterial speck. N.Z. J. Exp. Agric. 12, 161-164.
53. Rozsnyay, Zs.D., Hevesi, M., Klement, Z. and Vajna, L. 1992. Biological control against canker
and dieback diseases of apricots Acta Phytopathol. Entomol. Hung. 27, 551-556.
54. Ryder, M.H. and Jones, D.A. 1991. Biological control of crown gall using Agrobacterium
K84 and K1026. Aust. J. Plant Physiol. 18, 571-579.
55. Saad, S.M. and Hagedorn, .D.J. 1971. Control of bacterial brown spot of bean with a copper
fungicide. [Pseudomonas syringae
]. Plant Dis. Rep. 55: 735-737.
56. Sharma, S.L. 1981. Control of black rot of cauliflower by hot water seed treatment and field
sprays with streptocycline. Ind. J. Mycol. Plant Pathol. 11,17-20.
57. Sharon, E., Okon, Y., Bashan, Y. and Henis, Y. 1982. Detached leaf enrichment: a method for
detecting small numbers of Pseudomonas syringae
and Xanthomonas campestris
in seeds and symptomless leaves of tomato and pepper. J. Appl. Bacteriol. 53: 371-377.
58. Stall, R.E., and Thayer, P.L. 1962. Streptomycin resistance of the bacterial spot pathogen and
control with streptomycin. Plant Dis. Rprt. 46,389-392.
59. State of Israel. 1956. Compendium of plant protection regulation, Ministry of Agriculture,
60. Sundin, G.W., Demezas, D.H. and Bender, C.L. 1994. Genetic and plasmid diversity within
natural populations of Pseudomonas syringae
with various exposures to copper and streptomycin bactericides. Appl. Environ. Microbiol. 60, 4421-4431.
61. Tang, W.-H. 1994.Yield-increasing bacteria (YIB) and blocontrol of sheath blight of rice. In:
Improving plant productivity with rhizosphere bacteria. (Eds.) M.H. Ryder, P.M. Stephens and G.D. Bower. pp. 267-273. CSIRO Division of Soils, Australia.
62. Taylor, J.D., and Dudley, C.L. 1976. Seed treatment for the control of halo-blight of beans
). Ann. Appl. Biol. 85,223-232.
63. Tuzun, S., and Kloepper, J. 1994. Induced systemic resistance by plant growth-promoting
rhizobacteria. In: Improving plant productivity with rhizosphere bacteria. (Eds.) M.H. Ryder, P.M. Stephens and G.D. Bower. pp. 104-109. CSIRO Division of Soils, Australia.
64. Washington, W.S. 1991. Effect of Bordeaux mixture sprays applied afte flowering on fruit finish of
apricot. Plant Prot.Q. Victoria 6, 188-189.
65. Wilson, M. and Lindow, S.E. 1994. Ecological similarity and coexistence of epiphytic
icenucleating (Ice ) Pseudornonas syringae
strains and a non-ice-nucleating (Ice-) biological control agent. Appl. Environ. Microbiol. 60, 3128-3137.
66. Wimalajeewa, D.L.S., Cahill, R., Hepworth, G., Schnieder, H.G. and Washboume, J.W. 1991.
Chemical control of bacterial canker (Pseudomonas syringae
) of apricot and cherry in Victoria. Aust. J. Exp. Agric. 31, 705-708.
67. Yunis, H., Bashan, Y., Okon, Y. and Henis, Y. 1980. Weather dependence, yield losses and
control of bacterial speck of tomato caused by Pseudomonas tomato
. Plant Dis. 64,937-939.
Office of Environmental Health Hazard Assessment Proposition 65 No Significant Risk Levels (NSRLs) for Carcinogens and Maximum Allowable Dose Levels (MADLs) for Chemicals Causing Reproductive Toxicity Below is a list of NSRLs and MADLs that provide "safe harbor" for businesses subject to the requirements of Proposition 65. These NSRLs and MADLs are established in regulation in Tit
Acne is a skin condition seen as blackheads, whiteheads, pustules and inflamed and infected nodules found on theface, neck, chest and back. People with Acne often feel self-conscious about the blemishes on their This condition is found more in younger people. In some studies it has been found that as many as 80% teenagers are affected by Acne which can be caused by many factors. Acne can be